Nothing in life is to be feared, it is only to be understood.
Now is the time to understand more, so that we may fear less.
Training4CRM addresses gaps in Cell-based Regenerative Medicine (CRM) for treatment of neurodegenerative disorders (e.g. Parkinson’s (PD), Huntington’s (HD) and Epilepsy (EPI)), which occur as a result of progressive loss of structure, function and/or death of neurons in the brain. These disorders have a high prevalence and are associated with short- and long-term impairments and disabilities with high emotional, financial and social burden to the patients, their families and friends.
The Training4CRM consortium has produced a video to show and describe our work to the general public. You can find it here on Youtube (https://youtu.be/vF-aeKE34dE).
Our ESRs 2, 10 and 11 (Hakan, Theresa and Robin) are participating with the Training4CRM opto-electrical device in the Termis Business Plan Competition. They reached the final round and will pitch their business plan at the Termis conference in Rhodes 27.-31. May 2019.
To design, fabricate and characterise polymer and carbon based optoelectrical waveguides as future potential cell carrying implants with both optical and electrical properties.
To fabricate perfusable 3D hybrid scaffolds using novel hybrid 3D printing technology that simultaneously enables filament extrusion printing and hydrogel/bio-printing, incorporating in the same scaffold different material properties.
To fabricate various surface nanotopographies and microarchitectures in different materials, investigating their role in how cells make different developmental decisions.
Electronic circuits for optical stimulation, bioimpedance and electrochemical sensing.
To design, fabricate and characterise perfusion based bioreactor and sensor array systems for optimising and screening of 3D cell culture conditions and modelling neuronal disorders.
Perform linear and non-linear (memristive) properties of cells and tissues. To study the behaviour of the methods developed in some of the other ESRs in animal models.
To fabricate, characterise, apply 3D IPN scaffolds loaded with different ThFs as factor delivery systems (FDS) for improved hSC stability and induced differentiation.
Develop, characterise hSCs-producing ThFs, and autocrine/paracrine effects.
Develop human neurons releasing neurotransmitters of interest, either on the basis of a highly enriched population of one neuron subtype or by selectively stimulating the release from the desired population of neurons in vitro and/or in vivo.
To create a 3D microenvironment that allows scaling up of existing mini-brains towards “small brains”.
To create individualized blood vessels and brain networks in perfusable 3D scaffolds using de- and recellularization technology.
To study therapeutic factor releasing cell carrier implants in clinically relevant animal disease models.
To provide neurotransmitter releasing cell carrier implants in clinically relevant animal disease models (PD and HD).
Study the applicability of the GABA releasing cells with or without carrier implants in clinically relevant animal models of Epilepsy.
To design, fabricate and characterise a miniaturised instrument for monitoring both linear and non-linear (memristive) properties of cells and tissues, interfaced with the electrochemical sensor developed by ESR4.
Research School 1: Micro and Nanoengineering in Milano/Venice (6/11/2017 - 11/11/2017)Cross-disciplinary training of fellows in a range of different fast prototyping- and cleanroom based micro- and nanofabrication techniques to become knowledgeable and skilled in how to fabricate 3D structures in different materials, miniaturised 3D lab-on-a-chip systems and electronic components for wireless control and communication with the developed sensors and actuators, including system design and modelling. Transferable skills: Public engagement and responsible research and innovation.
Research School 2: Biotechnology in Madrid (29/4/2018 - 5/5/2018)Cross-disciplinary training of fellows in regenerative medicine, cell and gene therapy concepts, stem cell biology, and molecular and cellular biology methods to identify and characterise cellular phenotypes. Transferable skills: Sex and gender in science and continued public engagement.
Research School 3: Pre-clinical Studies and In Vivo Monitoring in Lund (25/9/2018 - 29/9/2018)Cross-disciplinary training of fellows in pre-clinical- and in vivo monitoring studies to become knowledgeable and skilled in how to deliver therapeutic factors and neurotransmitters in the healthy and diseased brain, monitor the therapeutic efficacy of cell based interventions in suitable models of nervous system disease, and design and conduct pre-clinical research as a means to prepare for future clinical trials. Transferable skills: Research management and research history.
Research school 4: Exploitation of Knowledge in Barcelona (TBA)Training of fellows in innovation management, IPR and exploitation of scientific knowledge and giving the fellows insights to the process of transforming scientific results into commercially viable solutions, incl. pitfalls and possibilities. The fellows will make posters on their innovation and business plan to be presented at our Training4CRM network conference (public event) scheduled for the end of the project.